Bacterial expression and characterization of starfish phospholipase A(2).

Bacterial Expression and Functional Characterization of A Naturally Occurring Exon6-less Preprochymosin cDNA

Chymosin (Rennin EC 3.4.23.4), an aspartyl proteinase, is the major proteolytic enzyme in the fourthstomach of the unweaned calf, and it is formed by proteolytic activation of its zymogene, prochymosin.Following the cloning of synthesized cDNAs on mRNA pools extracted from the mucosa of the calf fourthstomach, we have identified an alternatively spliced form of preprochymosin ...

P-84: Phospholipase C Zeta: As an Index to Assess Fertilization Potential of a Semen Sample

Background: Failed fertilization post ICSI has been mainly attributed to the sperm’s inability to induce oocyte activation. Phospholipase C Zeta (PLCζ) is considered to be a primary factor for the induction of oocyte activation. The aim of this study was to quantitatively assess the expression of PLCζ in globozoospermic or individuals with previous low or failed fertilization in comparison to f...

Isolation and characteristics of phospholipase A2 from the pyloric ceca of the starfish Asterina pectinifera

Evaluation of the effects of diethyl ether and methanol extracts of carpet anemone (Sarcophyton spp.) and starfish (Pentaceraster spp.) on E. coli and Bacillus subtilis bacteria

Marine environment is the main source of natural bioactive products whose chemical-structural properties are not similar with other natural products of plants and other inland organisms. The present study was carried out to evaluate antibiotic properties of diethyl ether and methanol extracts of two marine species including starfish and carpet anemone. The samples of starfish and carpet anemone...

Calcium Release at Fertilization in Starfish Eggs Is Mediated by Phospholipase Cγ

Although inositol trisphosphate (IP3) functions in releasing Ca2+ in eggs at fertilization, it is not known how fertilization activates the phospholipase C that produces IP3. To distinguish between a role for PLCgamma, which is activated when its two src homology-2 (SH2) domains bind to an activated tyrosine kinase, and PLCbeta, which is activated by a G protein, we injected starfish eggs with ...